Polypodium species of genus Polypodiaceae was investigated for preliminary phytochemical screening and microscopy. Different extracts were prepared with the help of hot extraction method by using soxhlet apparatus. Flavanoids, phytosterols, proteins, fatty acids and diterpenes were mainly detected. It was concluded that extracts of Polypodium decumanum contains important constituents which can be explored for the various pharmacological activities like antimicrobial, anthelmintic, antioxidant, anti-inflammatory etc. In microscopical examination, it was found that mainly multicellular trichomes, simple covering trichomes, fibres, epidermal cells, anomocytic stomata were present.
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